2008-10-01

Overexpression of ABI5 results in the plants being hypersensitive to abscisic acid such that they respond to low levels of abscisic acid which have no effect on wild type plants. One aspect of the invention is seeds, seedlings or plants which are resistant to drought because they overexpress ABI5.

Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. ABI5 overexpression also results in high sensitivity to glucose and anthocyanin accumulation in response to sugar stress (Finkelstein et al., 2002). Analyses of transcript accumulation in abi5 mutants suggest that, similar to ABI3, ABI5 has both activator and repressor functions that may have either synergistic or antagonistic effects on gene expression, depending on the target gene. Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions.

2020-03-24 Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 … 2020-11-07 2008-10-01 2020-03-02 2021-01-01 2019-02-01 Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas … ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP transcription factors of the AREB/ABF family, which promote ABA responses. AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis.

The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during

Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth. ABI5 overexpression also results in high sensitivity to glucose and anthocyanin accumulation in response to sugar stress (Finkelstein et al., 2002).

Overexpression of ABI5 in Col-0 influences flowering time To assess the correlation between flowering time and ABI5 expression, overexpressing transgenic lines carrying plas-mid p35S::ABI5-HA in Col-0 background were generated. A total of 43 lines were analysed by examining ABI5-HA protein level in individual transgenic plants (Fig. 1A).

ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP transcription factors of the AREB/ABF family, which promote ABA responses. AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants. The mRNA and protein level of ABI5 in overexpression plants on WT and mutant backgrounds were examined by quantitative real‐time PCR and immunoblotting, respectively.

By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYLs is also affected during the germination of abi5 mutants.
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These results indicate that HY5 inte-gratesbothABAandlightsignaltransductionpathwayspartially through direct activation of ABI5. Results HY5 Mediates ABA Response in Roots and Is Required for Osmotic The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked.

Our results suggest that ABA negatively regulates SHB1 expression, at least in part, through the action of its downstream signaling component ABI5.
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Overexpression of ABI5/ABF binding proteins (AFPs) results in extreme ABA resistance of seeds via multiple mechanisms repressing ABA response, including

Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Bar, 1 cm. (D,E) Fresh weight and root length of abi5-1, ABI5-HA overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 µM), and AZD (1 µM) for 10 days. Each graph 2016-12-20 Overexpression of ABI5 in plants to prevent precocious seed germination and to confer resistance to drought and high salt Mar 21, 2001 - The Rockefeller University The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants.